Silica Gel in SiliDispense(TM) Bot. 500-800mesh XtraFine for Col. Chromatography

Description: Silica gel for column chromatography, 500-800 mesh extra fine size. 400g / 0.88lb or 1.6kg / 3.53lbs SiO2 500-800 mesh (30-19µm particle size), pH 6-7, pore size 60Å Lot Experimental Data (Values subject to change based on lot, actual values will be labeled on bottle, however will not deviate too much)Density (loose)¹: 0.37g/cm³Density (well-packed)²: 0.44g/cm³Column volume³: 47mL/20gNormalized flow rate (2psi)⁴: 2.7mL/minSee bottle label for further description of each term. Not intended for long columns. Use a short, thick column. In fact, contrary to intuition, one should rarely ever need to use a long column. Because if a TLC plate of 6-7cm long can separate your compound, so should and can a column of ~10ish cm. A long column will only cause unnecessary peak broadening and high back-pressure. We care for you. No one should be exposed to silica dust. What causes the most harm may not be the most toxic, but the most common reagent due to exposure amounts. With our specially designed and packed SiliDispense(TM) bottles, say goodbye to the hassle and hazards of handling silica powder in your lab work. Easily twist open to uncap and squeeze out the silica directly into your column or final apparatus. You do not need to transfer from a large container to a secondary container. You do not need any tools or spatulas, while the SiliDispense(TM) bottles will fit in most lab spatulas if for any reason a spatula is to be used. Bottle size designed for benchtop storage and direct use. The sturdy, empty bottles can be reused for solvent storage and dispensing indefinitely. See photos and video demonstration for this great convenience and safe health feature. The following is an actual separation experiment performed with this silica: 15g of silica (500-800mesh) was added to a cotton-blocked glass chromatography column with I.D. 2.6cm(silica height ~8cm). Silica column was then wetted and packed using AAdvance’s in situ wet pack method (ref. AAdvance Instruments blog 06/2023). Column volume (lot) was 25mL. 0.2g dimethyl maleate (d.m.m.) and 0.2g dimethyl fumerate (d.m.f.) were loaded onto column as a suspension in 5% ethyl acetate in hexanes (4mL). Then, 10% ethyl acetate in hexanes was added as eluting solvent to start separation. Gentle pressurization of ~2psi was applied to column. Solvent was collected with test tubes with volumetric marks. The first fraction (d.m.f.) started eluting out at 65mL and finished eluting by 95-100mL. The second fraction (d.m.m.) started coming out at 115mL, and at the same time eluting solvent was replenished with 25% ethyl acetate in hexanes instead. Eluting finished at around ~150mL. See separation results by TLC spotting in image. This is a challenging separation, not only because of the compounds’ closeness in Rf and polarity, but also due to the fact that d.m.f. dissolves poorly in hexanes or 5% ethyl acetate in hexanes, causing peak broadening. Also available in: 200-300 mesh, 300-400 mesh

Price: 29.95 USD

Location: Stony Brook, New York

End Time: 2024-09-11T22:10:24.000Z

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