Description: Universal DNA/RNA extraction kit with magnetic beads. For mammalian tissue, bacterial, probiotics fungal, viral plants, and insects. 50T 1, Prepare solid samples a. Solid sample: take about 60 mg (maximum for lysis buffer 300 µl) of tissue. Homogenize it with 130 µl PBS. b. Liquid sample directly use 130 µl. If using a swab sample, 300 µl lysis buffer can be directly used to dissolve the samples. Solid samples µl Sample (PBS) 130 Lysis buffer 300 Proteinase K (10mg/ml Sigma) Not included in the kit 20 Magnetic beads (5mg/ml) 300 Isopropanol ( Not included in the kit) 450 Ethanol (70%) Not included in the kit 500 3, Add 300µl magnetic beads and mix well for 1 min. The binding buffer 450µl (100% Isopropanol) is added into the extraction mixture and rotated for 5 min at room temperature. 4, Set up the tube on the magnet and remove the supernatant. 5, Add 1 ml wash buffer (70% alcohol) and vortex for 10 seconds. Set up the tube on the magnet separation rack for 1-2 min and remove the supernatant. Repeat the step 3 times. 6, Set up the tube on the magnet rack for 2 min and completely remove the wash buffer. 7, Add 100 µl elution buffer (autoclaved ddH2O, or TE buffer) and set up on heat block for 3 min at 65 °C. 8, Collect eluted solution into a new autoclaved tube for future use. Note: 1, the Sample volume is adjustable. As long as keep a ratio. Sample:Lysis buffer:PK:Beads:Binding Buffer; 130:300:20:300:450. 2, If the test will use RNA of sample, make sure to prevent from RNase contamination in all solution. Liquid samples New ratio 400µl reaction 800µl reaction Lysis buffer 150 300 Magnetic beads (5mg/ml) 100 200 Isopropanol ( Not included in the kit) 150 300 wash buffer Ethanol (70%) Not included in the kit 750 1500 2, Directly dissolve the swab sample into 300 µl lysis buffer of 1 ml Eppendorf tube. Incubate for 30 min at 65 °C. Note: if a liquid sample, it needs about 5-10 min. 3, Add 200µl magnetic beads and mix well for 1 min. The binding buffer 300µl (100% Isopropanol) is added into the extraction mixture and rotate for 5 min at room temperature. 4, Set up the tube on the magnet and remove the supernatant. 5, Add 1 ml wash buffer (70% alcohol) and vortex for 10 seconds. Set up the tube on the magnet separation rack for 1-2 min and remove the supernatant. Repeat the step 3 times. 6, Set up the tube on the magnet rack for 2 min and completely remove the wash buffer. 7, Add 100 µl elution buffer (autoclaved ddH2O, or TE buffer) and set up on heat block for 3 min at 65 °C. 8, Collect eluted solution into a new autoclaved tube for future use. Note: 1, the Sample volume is adjustable. As long as keep a ratio. Sample:Lysis buffer:PK:Beads:Binding Buffer; 130:300:20:300:450. 2, If the test will use RNA of the sample, make sure to prevent RNase contamination in all solutions.
Price: 110 USD
Location: Pittsford, New York
End Time: 2024-09-24T13:37:40.000Z
Shipping Cost: 0 USD
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Item Specifics
Restocking Fee: No
Return shipping will be paid by: Seller
All returns accepted: Returns Accepted
Item must be returned within: 30 Days
Refund will be given as: Money back or replacement (buyer's choice)
Expiration Date: 12/20/2022
Brand: GeneNano
Custom Bundle: No
Model: Magnetic beads DNA extraction
MPN: Does Not Apply
Country/Region of Manufacture: United States
Modified Item: No
Intended Use/Discipline: research lab, Biological Laboratory, Cardiology, Emergency Medicine